Ambra de Simone - Biochemistry: Ascorbate extraction.
DescriptionI specialise in working and handling early germinated Arabidopsis seeds. I perform total and reduced ascorbate plate reader assay. In vivo glutathione redox potential measurements using redox-sensitive probes such as roGFP2 (redox sensitive GFP). I can also provide molecular biology services such as high-quality RNA extraction from Arabidopsis seeds.
I'm looking forward to working with you!
What do you need to provide us with?I perform ascorbate assay from frozen leaves tissues (minimum 80 mg).
For the extraction of RNA from seeds I would need 10-20mg of Arabidopsis seeds
What will I deliver?Ascorbate assays: Excel file with micromol per g FW calculated.
RNA extraction: 50 ul/10 mg of seeds of high-quality total RNA from dry seeds delivered on dry ice through the courier of your choice.